Abstract

Communication research traditionally prioritizes receiver variance, often overlooking how a single message induces commonality across distinct systems. Drawing on classical theory, we propose that effective messages entrain the biological rhythms of the audience. In a large-scale experiment (N=198) monitoring eye-tracking, heart rate, and electrodermal activity during a film, we mapped this alignment. Results reveal a processing hierarchy: alignment was strongest for information acquisition (gaze) but also extended to downstream autonomic regulation. Crucially, a temporal manipulation confirmed this state is content-locked: alignment vanished when the narrative sequence was altered but was restored upon computational unscrambling of the physiological time series. This confirms that responses carry a temporal fingerprint of the content. We conclude that messages function as alignment devices, reducing individual noise to create a network of receivers exhibiting shared processing states, thus providing a materially grounded, signal-based definition of the audience.